contoh perhitungan statistik validasi metode analisis

Lampiran 4.

Data hasil ketelitian antar analis pada media DG 18 untuk metode Rapid Y/M

Ulangan (n)	Jumlah koloni (cfu) duplo		Logaritma		Rata-rata (I)	Selisih (II)	(II/I)² (c)	Analis
	Plate 1 (a)	Plate 2 (b)	Log a	Log b
1	9	10	0,95424	1	0,97712	-0,0458	0,00219	A
2	9	9	0,95424	0,95424	0,95424	0	0	B
3	10	9	1	0,95424	0,97712	0,04576	0,00219	A
4	14	13	0,60206	1,11394	0,858	-0,5119	0,35593	B
5	12	12	1,07918	1,07918	1,07918	0	0	A
6	13	13	1,11394	1,11394	1,11394	0	0	B
7	15	15	1,17609	1,17609	1,17609	0	0	A
8	14	12	1,14613	1,07918	1,11265	0,06695	0,00362	B
9	10	10	1	1	1	0	0	A
10	8	6	0,90309	0,77815	0,84062	0,12494	0,02209	B
11	8	9	0,90309	0,95424	0,92867	-0,0512	0,00303	A
12	11	10	1,04139	1	1,0207	0,04139	0,00164	B
13	10	11	1	1,04139	1,0207	-0,0414	0,00164	A
14	8	8	0,90309	0,90309	0,90309	0	0	B
15	11	13	1,04139	1,11394	1,07767	-0,0726	0,00453	A
16	6	10	0,77815	1	0,88908	-0,2218	0,06226	B
17	9	11	0,95424	1,04139	0,99782	-0,0872	0,00763	A
18	12	11	1,07918	1,04139	1,06029	0,03779	0,00127	B
19	10	9	1	0,95424	0,97712	0,04576	0,00219	A
20	8	8	0,90309	0,90309	0,90309	0	0	B
Rata-rata	10,15		Σ				0,47024

Simpangan Baku Relatif(SBR) = √c/2*n = 0,108424

Koefisien Variansi=100*SBR  = 10.8424

Lampiran 5.     Data hasil keterulangan analis A

Ulangan (n)	Jumlah koloni (cfu) duplo		Logaritma		Rata-rata (I)	Selisih (II)	(II/I)² (c)
	Plate 1 (a)	Plate 2 (b)	Log a	Log b
1	9	10	0,954243	1,000000	0,977121	-0,04576	0,002193
2	10	9	1,000000	0,954243	0,977121	0,04576	0,002193
3	12	12	1,079181	1,079181	1,079181	0,00000	0,000000
4	15	15	1,176091	1,176091	1,176091	0,00000	0,000000
5	10	10	1,000000	1,000000	1,000000	0,00000	0,000000
6	8	9	0,903090	0,954243	0,928666	-0,05115	0,003034
7	10	11	1,000000	1,041393	1,020696	-0,04139	0,001645
8	11	13	1,041393	1,113943	1,077668	-0,07255	0,004532
9	9	11	0,954243	1,041393	0,997818	-0,08715	0,007628
10	10	9	1,000000	0,954243	0,977121	0,04576	0,002193
							0,023418

Simpangan Baku Relatif(SBR) = √c/2*n = 0,034218456

Koefisien Variansi=100*SBR = 3.4218456

Lampiran 6. Data hasil keterulangan analis B

Ulangan (n)	Jumlah koloni (cfu) duplo		Logaritma		Rata-rata (I)	Selisih (II)	(II/I)² (c)
	Plate 1 (a)	Plate 2 (b)	Log a	Log b
1	9	9	0,954243	0,954243	0,954243	0,000000	0,000000
2	14	13	0,602060	1,113943	0,858002	-0,511883	0,355931
3	13	13	1,113943	1,113943	1,113943	0,000000	0,000000
4	14	12	1,146128	1,079181	1,112655	0,066947	0,003620
5	8	6	0,903090	0,778151	0,840621	0,124939	0,022090
6	11	10	1,041393	1,000000	1,020696	0,041393	0,001645
7	8	8	0,903090	0,903090	0,903090	0,000000	0,000000
8	6	10	0,778151	1,000000	0,889076	-0,221849	0,062264
9	12	11	1,079181	1,041393	1,060287	0,037789	0,001270
10	8	8	0,903090	0,903090	0,903090	0,000000	0,000000
							0,446820

Simpangan Baku Relatif(SBR) = √c/2*n = 0,149469

Koefisien variansi=100*SBR = 14.9469

Lampiran 7. Data hasil akhir ketelitian antar analis

Ulangan (n)	Jumlah koloni (cfu) duplo		Logaritma		Rata-rata (I)	Selisih (II)	(II/I)² (c)
	Analis A	Analis B	Log a	Log b
1	9,5	9,0	0,977724	0,9542425	0,9659831	0,023481	0,000591
2	9,5	8,5	0,977724	0,9294189	0,9535713	0,048305	0,002566
3	12,0	13,0	1,079181	1,1139434	1,0965623	-0,034762	0,001005
4	15,0	13,0	1,176091	1,1139434	1,1450173	0,062148	0,002946
5	10,0	7,0	1,000000	0,845098	0,922549	0,154902	0,028193
6	8,5	10,5	0,929419	1,0211893	0,9753041	-0,091770	0,008854
7	10,5	8,0	1,021189	0,90309	0,9621396	0,118099	0,015067
8	12,0	8,0	1,079181	0,90309	0,9911356	0,176091	0,031565
9	10,0	11,5	1,000000	1,0606978	1,0303489	-0,060698	0,003470
10	9,5	8,0	0,977724	0,90309	0,9404068	0,074634	0,006299
							0,100555

Simpangan Baku Relatif(SBR) = √c/2*n = 0.0709066

Koefisien Variansi=100*SBR = 7.09066

Lampiran 8. Data akhir hasil ketelitian antar analis pada metode Rapid Y/M

Ulangan (n)	Jumlah koloni (cfu) duplo		Logaritma		Rata-rata (I)	Selisih (II)	(II/I)² (c)
	Analis A	Analis B	Log a	Log b
1	7	4	0,845098	0,602060	0,723579	0,243038	0,112818
2	5	9	0,698970	0,954243	0,826606	-0,255273	0,095370
3	6	5	0,778151	0,698970	0,738561	0,079181	0,011494
4	6	6	0,778151	0,778151	0,778151	0,000000	0,000000
5	9	11	0,954243	1,041393	0,997818	-0,087150	0,007628
6	5	10	0,698970	1,000000	0,849485	-0,301030	0,125576
7	7	10	0,845098	1,000000	0,922549	-0,154902	0,028193
8	5	5	0,698970	0,698970	0,698970	0,000000	0,000000
9	4	10	0,602060	1,000000	0,801030	-0,397940	0,246796
10	10	10	1,000000	1,000000	1,000000	0,000000	0,000000
							0,627874

Simpangan Baku Relatif(SBR) = √c/2*n = 0.1771827

Koefisien Variansi=100*SBR = 17.71827

Lampiran 9. Recovery relatif antar analis

Ulangan (n)	Jumlah koloni (cfu) duplo		Rapid Y/M
	Analis A	Analis B	Analis A	Analis B
1	9,5	9,0	7	4
2	9,5	8,5	5	8
3	12,0	13,0	6	4
4	15,0	13,0	6	6
5	10,0	7,0	9	9
6	8,5	10,5	5	9
7	10,5	8,0	7	10
8	12,0	8,0	5	5
9	10,0	11,5	4	10
10	9,5	8,0	10	10
Rata-rata recovery	10,7	9,7	6,4	7,5

Recovery Relatif analis A       = metode modifikasi/metode baku *100

                                     = 59,81308411

Recovery Relatif analis B        = metode modifikasi/metode baku *100

            = 73,52941176